Sulfate transport in Penicillium chrysogenum plasma membranes
نویسندگان
چکیده
منابع مشابه
Sulfate transport in Penicillium chrysogenum: cloning and characterization of the sutA and sutB genes.
In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products belong to the SulP superfamily of sulfate permeases were isolated. Transformation of a sulfate uptake-negative sB3 mutant of Aspergillus nidulans with the sutB gene completely restored sulfate upta...
متن کاملSulfate utilization by penicillin-producing mutants of Penicillium chrysogenum.
A great deal of work has been directed toward increasing the yield of penicillin in fermentations with Penicillium chrysogenum. The spectacular increase in yield which has been achieved has resulted, in the main, from two approaches. In the first approach, mutant strains of P. chrysogenum produced by spontaneous variation or by the action of mutagenic agents such as nitrogen mustard or ultravio...
متن کاملAccumulation of intracellular inorganic sulfate by Penicillium chrysogenum.
The cell membranes of microorganisms appear to be rather complex, metabolically active entities, equipped with many specific active transport mechanisms which mediate the uptake and accumulation of a variety of compounds. Bacteria have been shown to accumulate amino acids (Cohen and Rickenberg, 1956; Gale, 1947; 1953), carbohydrates (Cohen and Monod, 1957; Rickenberg et al., 1956), and inorgani...
متن کاملStructural and functional properties of plasma membranes from the filamentous fungus Penicillium chrysogenum.
Functional plasma membranes from the filamentous fungus Penicillium chrysogenum have been isolated with the objective of studying transport processes. The isolation procedure consists of three steps, namely homogenization of cells with a Braun MSK homogenizer, followed by Percoll gradient centrifugation and floatation of membranes in a three-step Nycodenz gradient. This method can be applied to...
متن کاملBasic amino acid transport in plasma membrane vesicles of Penicillium chrysogenum.
The characteristics of the basic amino acid permease (system VI) of the filamentous fungus Penicillium chrysogenum were studied in plasma membranes fused with liposomes containing the beef heart mitochondrial cytochrome c oxidase. In the presence of reduced cytochrome c, the hybrid membranes accumulated the basic amino acids arginine and lysine. Inhibition studies with analogs revealed a narrow...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1996
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.178.13.3953-3956.1996